It reacts specifically with -smooth muscle actin in immunoblotting assays and labels smooth muscle cells in frozen or formalin-fixed, paraffin-embedded tissue sections. Smooth muscle alpha actin is one of a few genes whose expression is predominantly expressed in vascular smooth muscle cells that is used to study myofibroblasts and fibrosis (1). Consistently, qPCR analyses revealed that these cells expressed high levels of cardiac transcription factors . Discover more for less. Histology and Immunohistochemistry. LAM is characterized by a nodular proliferation of smooth muscle-like cells in the peribronchial, perivascular, and perilymphatic lung tissue, accompanied by cystic dilation of the alveolus, rupture of the alveolar wall, lymphangiectasis, and septal collagen fiber deposition ( 19 ). Immunohistochemical staining of Smooth Muscle Actin (1A4) Monoclonal Antibody, on formalin fixed paraffin embedded human breast tissue at 1/50 dilution. alpha sma fibroblast marker. [Google Scholar] Five were also HHF-35 positive and three were desmin positive. We injected adipose-derived stem cells into mice to detect the wound healing effects and . Anti-alpha smooth muscle Actin Antibody (A82445) This product is identical to Abcam (ab21027). bell panovision shield; pirelli brand ambassador; grasp neo camo waterproof rain boot bogs; Noteworthy was strong positivity for the 'melanoma associated' antigen NKI/C3 in all cases. The antibody (also known as anti--Sm-1) is specific for the single isoform of -smooth muscle actin. Myofibroblasts are. Heterozygous De Novo and Inherited Mutations in the Smooth Muscle Actin (ACTG2) Gene Underlie Megacystis-Microcolon-Intestinal Hypoperistalsis Syndrome Sections were stained for Masson's trichrome, Verhoeff-van Gieson, TGF-1, CTGF, PDGF-B and smooth muscle myosin heavy chain 11 . BALB/C mice were injected with N-terminal decapeptide of -smooth muscle actin: Isotype : IgG2a,Kappa: State : Liquid: Storage: Store at 2C to 8C. Actin exists as a ubiquitous protein involved with filament formation that make up large portions of the cytoskeleton. Excessive accumulation of alpha smooth muscle actin-positive (ACTA2+) activated myofibroblasts is observed in Idiopathic pulmonary fibrosis (2). Steudel W. alpha-smooth-muscle actin and microvascular precursor smooth-muscle cells in pulmonary hypertension. View Mouse Monoclonal anti-alpha-Smooth Muscle Actin Antibody (SPM332) - IHC-Prediluted (NBP2-44462). The aim of the study was to evaluate the expression of the αSMA in patients with PDAC and CP as the possible . alpha Smooth Muscle Actin antibody Cat No. . KLH-conjugated synthetic peptide mapping to a fragment of residues within amino acids 343-371 in the C-terminal region of human ACTG2, UniProt Accession #NP_001606.1; P63267 . Nomura S. Identification, friend or foe: Vimentin and alpha-smooth muscle actin in cancer-associated . Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. Synthetic peptide corresponding to Human alpha smooth muscle Actin (N terminal).Database link: P62736 Validations None available Applications Enzyme-linked immunosorbent assay (ELISA) Flow cytometry/Cell sorting (FC/FACS) Immunocytochemistry (ICC) Immunocytochemistry-immunoflourescence (ICC-IF) Immunohistochemistry (IHC) custom hamburgers near me; best 15 inch off-road tires; integrated project delivery method pros and cons. This product ACTA2/smooth muscle actin, actin, alpha 2, smooth muscle, aorta, Rabbit, Polyclonal with code 23081-1-AP is available. It reacts specifically witha-smooth muscle actin in immunoblotting assays and labels smooth muscle cells in frozen or formalin-fixed, paraffin-embedded tissue sections. These antibodies should be outstanding resources to . -Smooth muscle actin (-SMA) is used as a marker for a subset of activated fibrogenic cells, myofibroblasts, which are regarded as important effector cells of tissue fibrogenesis. Description: The antibody (also known as anti-alpha-Sm-1) is specific for the single isoform of alpha-smooth muscle actin. Immunogen. As ezrin is a protein that links cell surface glycoprotein such as CD44H to the actin cytoskeleton and as in conventional osteosarcomas, -smooth muscle actin expression has been variably reported [ 10, 39, 42 ]; we also studied -muscle actin expression by immunohistochemistry (IHC) in the same specimens. Anaplastic lymphoma kinase (ALK-1) is expressed in approximately 40% of cases, and the tumor may express p80. Primary Antibody: Rabbit Polyclonal Antibody to Alpha Smooth Muscle Actin Abcam, Inc Cambridge, MA 02139 www.abcam.com 1-888-772-2226 Catalog # ab5694 Secondary Antibody: Alexa Fluor 568 Goat Anti-Rabbit IgG (H+L) *Highly Cross-Adsorbed* Life Technologies / Invitrogen Grand Island, NY 14072 www.invitrogen.com 1-888-584-8929 Catalog # A-11036 . We further confirmed the expression of the integrins in small PAs by immunohistochemistry, compared focal adhesion kinase (FAK) phosphorylation in PAs and PASMCs, . 100% Guarantee Price Match Guarantee Product Size: 10g Cells were used between passages 4 and 8. In our study, SM1, SM2, and SMemb were all positive in the normal media, as is the case in human teenagers . e-Labochema el. Pancreatic stellate cells (PSC) were isolated from surgically resected tumour tissue by the outgrowth method. Immunogen N-terminal synthetic decapeptide of -smooth muscle actin. Overview Specifications Images Highlighted for "alpha smooth muscle Actin Antibody" 95 View Datasheet Goat polyclonal antibody to alpha smooth muscle Actin for ELISA and WB. 2/1/2016 Review for Anti-alpha Smooth Muscle Actin Antibody Liyong Z. The main cells involved in fibrosis are pancreatic stellate cells (PSCs), which activate alpha smooth muscle actin (αSMA), which is considered to be the best-known fibrosis marker. Sanbio offers products from Proteintech. In particular, smooth muscle actin is an alpha actin that is found in skeletal muscle. With the progression of atherosclerosis, the expression of SM2 first decreases in the smooth-muscle cells in the intima followed by a decrease in the expression of SM1, whereas smooth-muscle -actin is well preserved . Recently Viewed SMA (Alpha Smooth Muscle Actin) $ 469.95 Products Downloads Products SMA-L-CE 1ml NCL-L-SMA In Stock Ships via Overnight Online-only price (details) $ 469.95 E. coli-derived recombinant human alpha -Actinin 1 . SMA (Alpha Smooth Muscle Actin) is recommended for the detection of specific antigens of interest in normal and neoplastic tissues, as an adjunct to conventional histopathology using non-immunologic histochemical stains. GTX100034 Reference 75 7 Datasheet File See all alpha Smooth Muscle Actin products APPLICATION Application Note *Optimal dilutions/concentrations should be determined by the researcher. Source. Materials and methods Alpha Smooth Muscle Actin (SMA) Immunohistochemistry Use in the Differentiation of Pancreatic Cancer from Chronic Pancreatitis Authors Katarzyna Winter 1 , Monika Dzieniecka 2 , Janusz Strzelczyk 3 , Magorzata Wgrowska-Danilewicz 4 , Marian Danilewicz 4 , Ewa Maecka-Wojciesko 1 5 Affiliations continental double fighter iii 700c; what are the 3 types of embroidery needles? Immunohistochemistry. Expiration: Stable for 6 months if correctly stored. Alpha-smooth muscle actin (alpha-SMA) is the actin isoform that predominates within vascular smooth-muscle cells and plays an important role in fibrogenesis. alpha-Smooth Muscle Actin Antibody Staining Protocol for Immunohistochemistry Description: ASM-1 represents an excellent marker for myogenic soft tissue tumours and smooth muscle differentiation. The samples were deparaffinized with xylol and ethanol followed by heat induced antigen retrieval with 10 mM citrate buffer. ab5694 reacts with tumors arising from smooth muscles and myoepithelial cells. A: Immunohistochemistry (IHC) of alpha-Smooth muscle actin (-SMA, prediluted mouse monoclonal anti- SMA, Abcam, Cambridge, UK) in a Stage IIIC colorectal carcinoma with high PRL-3. The expression of smooth muscle alpha-actin ( Acta2, which we abbreviate as alpha-SM-actin) and gamma-actin mRNAs and proteins was examined by real-time polymerase chain reaction and Western immunoblot, and was localized with immunohistochemistry, in the nonpregnant, pregnant, and postpartum rat uterus. It belongs to the actin family.It is predominantly found in cells of the smooth muscle lineages. IHC analysis of human colon using anti-alpha-Smooth Muscle Actin antibody. Not tested in other applications. Eng . They were also positive for the cardiomyocyte marker cardiac troponin T, the smooth muscle marker alpha smooth muscle actin or the fibroblast marker vimentin (Figure 3I, J). Smooth muscle alpha-actin (SMA) is the definitive marker for the activated cardiac fibroblast population known as the myofibroblast ( Frangogiannis et al., 2000 ), but this actin isoform is also abundant in vascular smooth muscle cells ( Skalli et al., 1989 ). In contrast, in . Smooth muscle actin is found on smooth muscle vessel walls, gut wall, myometrium, myoepithelial cells in breast and salivary glands. The specific NH2-terminal sequence Ac-EEED of alpha-smooth muscle actin plays a role in polymerization in vitro and in vivo. The antibody does not react with actin from fibroblasts, striated muscle, and myocardium. Alpha smooth muscle actin antibody (ab5694) stains smooth muscle cells in vessel walls, gut wall, and myometrium. The IHC staining and western blot revealed extracellular matrix (ECM) accumulation in penile cavernous tissues, and the smooth muscle cell (SMC) phenotypic transition was affected, as indicated by reduced alpha-smooth muscle actin and calponin-1 expression and increased phospho-myosin light chain 20 (p-MLC 20)/MLC 20 and osteopontin expression . Calculated MW 42 kDa. Description Cytoplasmic actins, which belong to the microfilament system of cytoskeleton proteins, are some of the most conserved eukaryotic proteins being expressed in mammals and birds. . Neointima formation in TV is the accumulation of -smooth muscle actin (-SMA)-positive smooth muscle cells (SMCs) between the endothelial lining and the internal elastic lamina (IEL), . Validated Applications: IHC, IHC-P. Validated Species: Human, Mouse, Rat + more. The sample well of each lane was loaded with 50ug of sample under reducing conditions. A fluorescence microscope is an optical microscope that uses fluorescence instead of, or in addition to, scattering, reflection, and attenuation or absorption, to study the properties of organic or inorganic substances. Actin Alpha Smooth Muscle, validated in IHC (ADB1001-1), Abcepta. IHC was performed on formalin fixed paraffin embedded sections. Monoclonal Mouse IgG 2B Clone # 910301 . Protein A or G purified from hybridoma culture supernatant . The expression of nuclear proliferation antigen ki67 and -SMA in tumor tissues of each group was analyzed by immunohistochemistry. Detects human alpha -Actinin 1 in ELISA, and human, mouse and rat alpha -Actinin 1 in Western Blot. ab195889 Anti-alpha Tubulin antibody Immunocytochemistry/ Immunofluorescence abreview for Anti-PD-L1 antibody [28-8] Excellent. Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-alpha smooth muscle Actin antibody [ACTA2/791] (ab220179) Immunohistochemical analysis of formalin fixed, paraffin embedded Human colon carcinoma tissue labeling alpha smooth muscle Actin with ab220179 at 0.5 g/ml. The dissected subcutaneous tumor tissues were fixed using 4% paraformaldehyde. Immunohistochemistry and immunofluorescence was performed on slides from whole sections of tissue blocks using antibodies against COX-2 and -smooth muscle actin (SMA). parduotuv: cheminiai reagentai, ranga ir prietaisai laboratorijoms, baldai IMMUNOHISTOCHEMISTRY Immunohistochemical stains for vimentin, smooth muscle actin, and muscle-specific actin are generally positive. Immunogen. Actin filaments interact with myosin to assist in muscle contraction as well as aiding in cell motility and cytokinesis. All cases showed at least focally the light microscopic features of a smooth muscle tumour and demonstrated moderate to strong positivity for alpha-smooth muscle actin. Am J Respir Cell Mol Biol. Aim: Fibrosis is observed both in pancreatic cancer (PDAC) and chronic pancreatitis (CP). Smooth muscle actin reacts with the alpha-smooth muscle isoform of actin, and labels the smooth muscle cells of vessels, myoepithelial cells, pericytes, some stromal cells in the intestine, testis, and ovary, and tumors derived from smooth muscle cells. After preparation the tissue was blocked with normal serum for 20 min at RT. ( Note ) Positive Control Western blot analysis of Alpha-Smooth Muscle Actin using anti-Alpha-Smooth Muscle Actin antibody (MA1106). View specifications, prices, citations, reviews, and more. Defects in the smooth muscle actin gene cause aortic aneurysm familial thoracic type 6. In the ovary with its cyclically developing and regressing functional bodies and the associated intense neovascularisation and remodelling, alpha-smooth muscle actin (SMA) immunolocalisation has been frequently used as a marker to establish vessel hierarchy, in angiogenesis studies, or in studies characterising ovarian neoplasms in various species. Myoepithelial cells in breast and salivary gland are also stained. Actin is a 43000 kDa ubiquitous protein found in all cells Actins are involved in cell motility (alpha, smooth muscle) and the maintenance of the cytoskeleton (beta and gamma, all cells) Antibodies to alpha smooth muscle actin do not detect the other actin isoforms Essential features Involved in cell motility -smooth muscle actin, a smooth muscle cell-specific protein, is also known as aortic smooth muscle or a smooth muscle actin (-SMA, SMactin, -SM-actin, ASMA). Primary Antibody Please apply for a account to purchase this product online. Tested applications Suitable for: WB, IHC-P more details Species reactivity SMA (Alpha Smooth Muscle Actin) is recommended for the detection of specific antigens of interest in normal and neoplastic tissues, as an adjunct to conventional histopathology using non-immunologic histochemical stains. Gender-Specific Coagulation Profiles of Peripheral and Portal Blood May Help to Differentiate Malignant from Benign Pancreatic TumourPilot Study Research Areas. Lane 1: human placenta tissue lysates, 1999; 20:582-594. . Purification. Immunohistochemistry. . Compare Anti-alpha-Smooth Muscle Actin Immunohistochemistry Antibody Products from leading suppliers on Biocompare. Download Citation | On Oct 26, 2022, Pilar Cossio and others published Catching actin proteins in action | Find, read and cite all the research you need on ResearchGate Actin filaments interact with myosin to assist in muscle contraction as well as aiding in cell motility and cytokinesis. The validation in immunohistochemistry using rat tissues clearly demonstrates specific labeling of different subsets of cells by the isoform-specific antibodies.
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