Highly specific and rigorously validated in-house, Rab7 (D95F2) XP Rabbit Monoclonal Antibody (CST #9367) is ready to ship. ADP-GloTM kinase assay kit was purchased from Promega. Depositors Institution: MRC Laboratory of Molecular Biology Rab7 dimer was concentrated to 6 mg/mL using a 10-kDa-molecular-weight cutoff centrifugal concentrator (Amicon). Purity: Greater than 90 % as determined by reducing SDS-PAGE. Dextran blue (405) and dextran Alexa Fluor 488 (molecular weight, 10,000), Alexa Fluor 647conjugated EGF, and LysoTracker red DND-99 were purchased from Life Technologies. Z.-X., Feng, G.H., Raposo, G., and Spritz, R.A. (2000). Cellular distribution: Plasma membrane, cytosol, The study of EVs has the potential to identify unknown cellular and molecular mechanisms in intercellular communication and disease. The rab5 protein plays an important regulatory role in early endocytosis, yet the function of rab7 protein was previously uncharacterized. RAB7A (a.k.a. Rab5 and rab7 proteins belong to a superfamily of small molecular weight GTPases known to be associated with early and late endosomes, respectively. Epub 2010 Sep 21. It #: 10135 Product Name: Rab7 Protein Q67L mutant Synonyms: Member RAS oncogene family, Rab7a, MGC102153 Source: Human, recombinant full length, His6-tag Expression Host: E. coli Molecular Weight: 23 kDa Purity: >95% by SDS-PAGE Introduction: Members of the Rab family of Ras-related GTP-binding proteins are important regulators of vesicular transport and are Pulmonary edema occurs in settings of acute lung injury, in diseases, such as pneumonia, and in acute respiratory distress syndrome. Small GTPase, Rab7 is known to recruit retromer on endosomal membrane via interactions with the Vps sub-complex. Immunoprecipitation using anti-ubiquitin antibodies followed by RAB7 immunoblotting yielded RAB7 bands of higher molecular weight, revealing ubiquitin modification (Fig. Rab7 associates with the RIPL effector protein to control membrane trafficking from early to late endosomes and to lysosomes (2,3). Rab7 also helps to regulate growth receptor endocytic trafficking and degradation (3,4), and maturation of phagosome and autophagic vacuoles (4-6). A high through-put screen of the Molecular Libraries Small Molecule Repository (MLSMR) identified a compound, ML282, which potently inhibits GTPases from all three subfamilies. The small molecular weight G-protein RAB7 is localized to both early and late endosomes and has been shown to be critical for trafficking through the endocytic Overexpression of wild-type rab7 protein allowed normal transport to late endosomes (mannose 6-phosphate receptor positive), while the rab7N125I mutant caused the VSV G protein to accumulate specifically in early (transferrin receptor positive) endosomes. This strain may be useful for Distribution Tissue distribution: Rab7A is ubiquitously expressed. CMT2B, PRO2706, RAB7) Promoter CMV Tag / Fusion Protein. During concentration, the buffer was exchanged to Available in 2 sizes. Catalog. Appearance: Lyophilized powder. In this study, previously published Rab7 sequences from National Center for Biotechnology Information (NCBI) have been investigated from chordates, mollusks, annelids, cnidarians, amphibians, priapulids, brachiopods, and arthropods including decapods and other groups. The molecular machinery behind lysosome biogenesis and the maintenance of the perinuclear aggregate of late endocytic structures is not well understood. A likely candidate for being part of this machinery is the small GTPase Rab7, but it is unclear whether this protein is associated with lysosomes or plays any role in the regulation of the perinuclear lysosome Formulation: Lyophilized a 0.2 m filtered solution of Molecular Weight: 390.48. Mouse Rab7 (19349), fruit fly Rab7 (42841), Zebrafish rab7 (393902) Related Products: Antibodies esiRNA shRNA siRNA. Exosomes, with an average diameter of ≈100 nanometers, are a subset of EVs. Molecular Biology It is a short 16.3 kDa protein containing several highly conserved regions, including the transmembrane segment, the ligand-binding domain, and flexible segments at the N terminus and the C terminus of the protein crucial for Extracellular vesicles (EVs) are nanoscale membrane vesicles released by donor cells that can be taken up by recipient cells. PcRab7 was widely expressed in various tissues including haemocytes, Mouse Rab7 (19349), fruit fly Rab7 (42841), Zebrafish rab7 (393902) Related Products: Antibodies esiRNA shRNA siRNA. Wang T, Ming Z, Xiaochun W, Hong W: Rab7: role of its protein interaction cascades in endo-lysosomal traffic. The rab5 1, bottom two rows), Work is in progress to identify the molecular motor(s) controlling transport and to discern the underlying regulation. Molecular Weight: 390.48. 2. The predicted molecular weight and isoelectric point of PcRab7 were 23.2 kDa and 5.77, respectively. The molecular mechanism underlying the recruitment process including the role of individual Vps proteins is yet to be deciphered. Rab7 (or Ypt7 in yeast) is one of the well-characterized members of the Rab family small GTPases, which serve as master regulators of membrane trafficking in eukaryotes. This antibody is specific to RAB7A. Cytoplasmic PCNA is located in the actin belt and involved in osteoclast differentiation. FV-429 induces autophagy blockage and lysosome-dependent cell death of T-cell malignancies via lysosomal dysregulation. Compare Product No. Protocol 26473: Standard PCR Assay - Rab7 Version 1. Cat. 2011 Mar;23(3):516-21. doi: 10.1016/j.cellsig.2010.09.012. Educational Resources. The rab5 protein (50.2%), mean molecular weight and isoelectric point of 23.269 kDa and 5. Anti-RAB7 Antibodies | Invitrogen - Thermo Fisher Scientific Compare Product No. Cited in 373 publications. CMT2B, PRO2706, RAB7) Promoter CMV Tag / Fusion Protein. RAB7A (a.k.a. Through their effector proteins, Rab GTPases regulate vesicle formation, actin and tubulin-dependent vesicle movement, and membrane fusion. Abstract. The compound behaves as a competitive inhibitor of Rab7 nucleotide binding based on both equilibrium binding and dissociation assays. Molecular docking analyses are compatible with CID 1067700 fitting into the nucleotide binding pocket of the GTP-conformer of Rab7. Rab7 regulates vesicle traffic from early Although the chimeras between GFP and the dominant negative forms of Rab7 (T22N and N125I) were not obviously colocalized with internalized dextran in late endocytic compartments (Fig. FD Rapid Golgi staining kit was purchased from FD Neurotechnologies. Validated for WB, IP, IF. Description SDS Pricing; 553326: The Ras Superfamily GTPases GTP/GDP Binding Antagonist, CID 1067700 controls the biological activity of Rab7. Rab7 and Rab34 are implicated in regulation of lysosomal morphology and they share a common effector referred to as the RILP (Rab-interacting lysosomal protein). Rab5 and rab7 proteins belong to a superfamily of small molecular weight GTPases known to be associated with early and late endosomes, respectively. Cell Signal. S3D). Rab7A is a 207 amino acid protein with a molecular mass of 23 kD. What is the molecular weight of TOM20? 021589 B6.129(Cg)- Rab7 tm1.1Ale /J These Rab7 flox mutant mice possess loxP sites flanking exon 1 of the RAB7, member RAS oncogene family ( Rab7 ) gene. Rab5 and rab7 proteins belong to a superfamily of small molecular weight GTPases known to be associated with early and late endosomes, respectively. Select a category to narrow your search. Calculated molecular weight: 23 kDa: Observed molecular weight: 23 kDa: GenBank accession number: NM_004637: Gene symbol: RAB7A: Gene ID (NCBI) 7879: Conjugate: Unconjugated: Form: Liquid: Purification Method: Antigen Abstract. Abstract. Notes. A Monoclonal Antibody for studying Rab7. Rab7 is the characteristic Rab marker of late endosomes . Molecular Weight: Approximately 25.7 kDa. Molecular Weight 23489.55 Theoretical pI Not Available GO Classification. We found 113 results for: Rab7. Different molecular families have Rab5 and rab7 proteins belong to a superfamily of small molecular weight GTPases known to be associated with early and late endosomes, respectively. 1 in Blog Posts. Rab5 and rab7 proteins belong to a superfamily of small molecular weight GTPases known to be associated with early and late endosomes, respectively. The rab5 protein plays an important regulatory role in early endocytosis, yet the function of rab7 protein was previously uncharacterized. The genotyping protocol(s) presented here have been optimized for reagents and conditions used by The 1 in Collections. Ras-related protein Rab-7a; Ras-related protein BRL-RAS; RAB7A; RAB7. mCherry (N terminal on backbone) Cloning Information Cloning method Unknown 5 sequencing primer CMV-F 3 The mammalian small molecular weight GTPase Rab7 (Ypt7 in yeast) has been implicated in regulating membrane traffic at postinternalization steps along the endosomal pathway. The rab5 protein plays an 111 in Plasmids. mCherry (N terminal on backbone) Cloning Information Cloning method Unknown 5 sequencing primer CMV-F 3 sequencing primer SV40pA-R
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